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human breast cancer cell line mcf 7  (ATCC)


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    ATCC human breast cancer cell line mcf 7
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf+7+breast+cancer+cells/MCF7/pm42874407-88-1-7
    Average 99 stars, based on 35160 article reviews
    human breast cancer cell line mcf 7 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: TGF-α/EGFR-mediated lymphatic metastasis reveals a repositionable therapeutic target in breast cancer.
    Article Snippet: 4T1 cells were from ATCC (American Type Culture Collection, VA) and cultured in RPMI and supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific) and 1% penicillin + streptomycin. .. Mouse lymphatic endothelial cells (svLEC) (provided by Dr Jonathan S. Alexander, AR TI CL E IN P RE SS Louisiana State University Health Sciences Center, Shreveport, Louisiana), and human MDA-MB231 and MCF-7 breast cancer cells (both from ATCC) were cultured in DMEM with high glucose supplemented with 10% FBS and 1% penicillin–streptomycin. ..

    Article Title: TGF-α/EGFR-mediated lymphatic metastasis reveals a repositionable therapeutic target in breast cancer
    Article Snippet: 4T1 cells were from ATCC (American Type Culture Collection, VA) and cultured in RPMI and supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific) and 1% penicillin + streptomycin. .. Mouse lymphatic endothelial cells (svLEC) (provided by Dr Jonathan S. Alexander, Louisiana State University Health Sciences Center, Shreveport, Louisiana), and human MDA-MB-231 and MCF-7 breast cancer cells (both from ATCC) were cultured in DMEM with high glucose supplemented with 10% FBS and 1% penicillin–streptomycin. ..

    Cell Culture:

    Article Title: TGF-α/EGFR-mediated lymphatic metastasis reveals a repositionable therapeutic target in breast cancer.
    Article Snippet: 4T1 cells were from ATCC (American Type Culture Collection, VA) and cultured in RPMI and supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific) and 1% penicillin + streptomycin. .. Mouse lymphatic endothelial cells (svLEC) (provided by Dr Jonathan S. Alexander, AR TI CL E IN P RE SS Louisiana State University Health Sciences Center, Shreveport, Louisiana), and human MDA-MB231 and MCF-7 breast cancer cells (both from ATCC) were cultured in DMEM with high glucose supplemented with 10% FBS and 1% penicillin–streptomycin. ..

    Article Title: TGF-α/EGFR-mediated lymphatic metastasis reveals a repositionable therapeutic target in breast cancer
    Article Snippet: 4T1 cells were from ATCC (American Type Culture Collection, VA) and cultured in RPMI and supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific) and 1% penicillin + streptomycin. .. Mouse lymphatic endothelial cells (svLEC) (provided by Dr Jonathan S. Alexander, Louisiana State University Health Sciences Center, Shreveport, Louisiana), and human MDA-MB-231 and MCF-7 breast cancer cells (both from ATCC) were cultured in DMEM with high glucose supplemented with 10% FBS and 1% penicillin–streptomycin. ..

    Polymer:

    Article Title: Development of an Anticancer Fungal Paclitaxel-Loaded Hydrogel System via 3D Bioprinting: A Next-Generation Biomedical Platform
    Article Snippet: .. The cytotoxicity of the polymer, drug, and polymer-drug combination was evaluated using the MTT assay on MCF-7 breast cancer cells., The ATCC provided the MCF-7 cells, which were then cultivated in DMEM media enhanced with 10% fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 μg/mL streptomycin. ..

    MTT Assay:

    Article Title: Development of an Anticancer Fungal Paclitaxel-Loaded Hydrogel System via 3D Bioprinting: A Next-Generation Biomedical Platform
    Article Snippet: .. The cytotoxicity of the polymer, drug, and polymer-drug combination was evaluated using the MTT assay on MCF-7 breast cancer cells., The ATCC provided the MCF-7 cells, which were then cultivated in DMEM media enhanced with 10% fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 μg/mL streptomycin. ..



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    Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and <t>ER+</t> <t>MCF-7</t> cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.
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    Siglec-10 binding to R3 and derivatives. (a) Structures of glycans R1 to R5. (b) Glycan array results of Siglec-10- Fc binding to glycans R1 to R5. Data are mean ± SD (c) Flow cytometry results of Siglec-10 binding to <t>MCF-7</t> in the presence of R1 to R5 (200 μM), respectively. (d) Siglec-10 binding to MCF-7 at different R3 concentrations. (e) Siglec-10 binding to MCF-7 at different R5 concentrations.
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    Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Quantitative Proteomics, Knockdown, Expressing, Quantitative RT-PCR, Control

    Siglec-10 binding to R3 and derivatives. (a) Structures of glycans R1 to R5. (b) Glycan array results of Siglec-10- Fc binding to glycans R1 to R5. Data are mean ± SD (c) Flow cytometry results of Siglec-10 binding to MCF-7 in the presence of R1 to R5 (200 μM), respectively. (d) Siglec-10 binding to MCF-7 at different R3 concentrations. (e) Siglec-10 binding to MCF-7 at different R5 concentrations.

    Journal: Journal of the American Chemical Society

    Article Title: A Common Small-Molecule Inhibitor Targeting Multiple Siglecs Associated with Immune Checkpoint Suppression of Cytotoxicity and Phagocytosis

    doi: 10.1021/jacs.6c15475

    Figure Lengend Snippet: Siglec-10 binding to R3 and derivatives. (a) Structures of glycans R1 to R5. (b) Glycan array results of Siglec-10- Fc binding to glycans R1 to R5. Data are mean ± SD (c) Flow cytometry results of Siglec-10 binding to MCF-7 in the presence of R1 to R5 (200 μM), respectively. (d) Siglec-10 binding to MCF-7 at different R3 concentrations. (e) Siglec-10 binding to MCF-7 at different R5 concentrations.

    Article Snippet: Human breast cancer cell lines MCF-7 (HTB-22, the American Type Culture Collection, WT) and CD24-knockout MCF-7 (KC-1753, KYinno Biotechnology, CD24-KO) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 1 × nonessential amino acids (Gibco) and 1 × antibiotic-antimycotic (Gibco).

    Techniques: Binding Assay, Glycoproteomics, Fc Binding, Flow Cytometry

    In vitro phagocytosis assay with flow cytometry. (a) Phagocytosis of M0-like, M1-like, or M2-like (IL-10-/IL-4-induced) macrophages to MCF-7. Normalized phagocytosis of (b) M0-like, (c) M1-like, (d) M2-like (IL-10-induced), and (e) M2-like (IL-4-induced) macrophages. Phagocytoses were normalized to the highest value of each experiment ( n = 3 donors). (f) Cytotoxicity of NK cells to MCF-7 in the presence of 2 mM R3 or PBS control. Cytotoxicity activities were normalized to the highest value of each experiment ( n = 3 donors). Statistical significance was determined using a paired, two-tailed t test on the original raw data across independent biological replicates ( n = 3 donors; * p < 0.05, ** p < 0.01) Data are mean ± SEM.

    Journal: Journal of the American Chemical Society

    Article Title: A Common Small-Molecule Inhibitor Targeting Multiple Siglecs Associated with Immune Checkpoint Suppression of Cytotoxicity and Phagocytosis

    doi: 10.1021/jacs.6c15475

    Figure Lengend Snippet: In vitro phagocytosis assay with flow cytometry. (a) Phagocytosis of M0-like, M1-like, or M2-like (IL-10-/IL-4-induced) macrophages to MCF-7. Normalized phagocytosis of (b) M0-like, (c) M1-like, (d) M2-like (IL-10-induced), and (e) M2-like (IL-4-induced) macrophages. Phagocytoses were normalized to the highest value of each experiment ( n = 3 donors). (f) Cytotoxicity of NK cells to MCF-7 in the presence of 2 mM R3 or PBS control. Cytotoxicity activities were normalized to the highest value of each experiment ( n = 3 donors). Statistical significance was determined using a paired, two-tailed t test on the original raw data across independent biological replicates ( n = 3 donors; * p < 0.05, ** p < 0.01) Data are mean ± SEM.

    Article Snippet: Human breast cancer cell lines MCF-7 (HTB-22, the American Type Culture Collection, WT) and CD24-knockout MCF-7 (KC-1753, KYinno Biotechnology, CD24-KO) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 1 × nonessential amino acids (Gibco) and 1 × antibiotic-antimycotic (Gibco).

    Techniques: In Vitro, Phagocytosis Assay, Flow Cytometry, Control, Two Tailed Test